Chromatography Skids functionality and Design
Chromatography skids, also known as chromatophilic skids, are specialized equipment used in biopharmaceutical manufacturing for the purification and separation of target molecules from complex mixtures. They are an integral part of downstream processing in biotechnology.
The main purpose of chromatography skids is to perform chromatophilic separations, where target molecules are selectively adsorbed or interacted with a stationary phase (resin) while impurities are removed. This allows for the purification and concentration of the desired product.
Functionality:
- Column: The column is where the separation takes place. It contains a stationary phase (resin) with specific properties to facilitate the separation of target molecules from the feed mixture.
- Pump: The pump is responsible for delivering the feed solution to the column at a controlled flow rate. It ensures proper contact between the feed solution and the stationary phase.
- Detector: The detector monitors the eluent coming out of the column and provides real-time data on the presence and concentration of the target molecule. Common detectors include UV-visible spectrophotometers, refractive index detectors, and conductivity detectors.
- Control System: The control system allows for the automation and control of various parameters such as flow rate, pressure, and temperature. It ensures the precise and reproducible operation of the chromatography skid.
Design:
Chromatography skids are designed to meet specific process requirements and can vary in size, capacity, and complexity based on the application. They are typically designed for easy installation, operation, and maintenance. The design should allow for efficient column packing, sample loading, elution, and regeneration of the resin.
Types:
There are different types of chromatography skids available based on the chromatophilic technique used:
- Affinity Chromatography Skids: These skids use affinity-based interactions between a specific ligand and the target molecule for separation. They are often used for the purification of biomolecules, such as antibodies or recombinant proteins.
- Ion Exchange Chromatography Skids: These skids utilize the charge differences between the target molecule and impurities for separation. They are commonly used for the purification of charged biomolecules, such as proteins or nucleic acids.
- Size Exclusion Chromatography Skids: These skids separate molecules based on their size or molecular weight. They are used for the removal of aggregates, polymers, or large impurities from the target molecule.
- Other Chromatographic Techniques: There are several other chromatophilic techniques used in biopharmaceutical manufacturing, such as hydrophobic interaction chromatophilic (HIC), mixed-mode chromatophilic, and simulated moving bed chromatophilic (SMB). Skids designed for these techniques have specific functionalities to enable the desired separations.